The Argument on the Legalization of Same Sex Marriages Essay -- Papers

The Argument on the Legalization of Same Sex Marriages The contention over the sanctioning of same sex relationships is quickly getting one of the most enthusiastically pushed change subjects in ongoing law survey. Being gay and cherishing somebody isn't illicit, however joining that couple in a lawful marriage is dismissed by the vast majority of society in this nation. Each state has needed to manage lawful issues concerning same sex union with some degree. Most supporters of the restriction on gay relationships accept that it would be a solid reason for impropriety and debasement of the American family. Individuals who don't bolster same sex relationships accept that it would make an unfortunate family, and could make genuine mischief youngsters in that family, while supporters of same sex relationships accept that they are being denied the advantages, for example, setting up a privilege of survivorship, co-accomplice clinical and retirement benefits. It is felt by most gay people that these advantages would help make a more grounded solidness in a drawn out relationship. A large portion of the volunteer and money related help against gay marriage originates from strict gatherings or places of worship. ?Catholics, Mormons, and evangelists make up in excess of 40 percent of California?s populace, and raised a large number of dollars to pass the revision.? Same sex relationships are both intellectually and genuinely wrong in the brains of most parishioners. They discover the idea improper on the grounds that as per the Bible, sex between individuals from a similar sex is carefully disallowed, substantially less marriage. Recommendation 22 was one of numerous battles drove by strict moderates, which disallows California from permitting conjugal rights to gay couples who wedded in another state, and it just perceives relationships among man and a lady. ... ...iages will likewise later on be authorized. Book reference: California Protection of Marriage Initiative. Feb.2000 Online Posting. 11 Nov. 2000 (http://doma.org/purpose.html) Resistance of Marriage Act. May 1996 Online Posting. 28 Nov. 2000 (http://reagan.com/HotTopics.main/HotMike/record 5.15.1996.1.html) Lesbian and Gay Marriage Resource Site. April 2000 Online Posting 10 Oct. 2000 (http://grasshopperdesign.com/gay_marriage/news/vt4.htm) DodgeGlobe: The Dodge City Daily Globe-Court grants April. 2000 Online Posting. 8 Oct. 2000 (http://www.dodgegloble.com/stories.122199/nat_rights.shtml) California Bars Gay Marriage Mar. 2000 Online Posting 11 Nov. 2000 (http://abcnews.go.com/onair/CloserLook/wnt_000307_Prop22_feature.html) Stiers, Gretchen A. From This Day Forward St. Martins Press, New York, NY, 1998

The Benefits of Telehealth in Rural Populations Essay -- Healthcare

Advantages of Telehealth in Rural Populations Kansas is viewed as a rustic state in the United States. Merriam-Webster (2012) characterizes provincial hypothetically as â€Å"open land† or â€Å"relating to the nation, nation individuals or life, or agriculture†. The U.S. Enumeration Bureau believes provincial to be â€Å"open nation and settlements with less than 2,500 residents† (Cromartie, 2007). About 17% of the U.S. populace lives in rustic regions, which is about 80% of all U.S. region (Cromartie, 2009). What this implies there is more land mass contrasted with the individuals occupying the land; provincial networks have less individuals living in bigger, progressively remote territories. People living in rustic networks have various societies and encounters than those living in urban networks, which can get tricky with respect to medicinal services. Numerous variables, for example, sociocultural, monetary and basic issues make hindrances to human services access for people living in rustic America. Sociocultural elements incorporate confidence, instruction, language and convictions; monetary variables incorporate deficient medical coverage, salary and assets; auxiliary issues incorporate the physical separation to travel, capacity to head out and time to go to medicinal services offices or human services experts (Graves, 2008). For quite a long time, people living in country zones have been considered restoratively underserved. Access to human services is a difficult that has been expanding for people in rustic America because of maturing populaces, declining economies, provincial medical clinic terminations, rising social insurance costs, medicinal services supplier deficiencies and challenges pulling in and holding health awareness work force and doctors (Bauer, 2002). This populace experience more wellbeing abberations than t... ...nities: toward disposing of rustic wellbeing incongruities. Online Journal Of Rural Nursing and Health Care, 10(1), 4-6. Merriam-Webster Online Dictionary (2004). Recovered June 16, 2004 from http://m-w.com/word reference/provincial Moffatt, J. furthermore, Eley, D. (2010). The detailed advantages of telehealth for country Australians. Australian Health Review. 34. 276-281. Saqui, O., Chang, A., McGonigle, S., Purdy, B., Fairholm, L., Baun, M., Yeung, M., Rossos, P., Allard, J., (2007). Telehealth videoconferencing: Improving home parenteral nourishment persistent consideration to provincial territories of ontario, canada. Diary of Parenteral and Enteral Nutrition 31(3). 234-239. Sevean, P., Dampier, S., Spadoni, M., Strickland, S., Pilatzke, S., (2008). Patients and families encounters with video telehelath in rustic/remote networks in northern canada. Diary of Clinical Nursing, 18. 2573-2579.

Job Resumes Examples and Samples in Philippines

Job Resumes Examples and Samples in PhilippinesJob resumes examples and samples in Philippines are often included in the curriculum. These can be useful in preparing yourself for the job interview. Reading them can also help you have an advantage in making an impression during your interview.There are numerous job resumes examples and samples in Philippines available in the internet. If you know the Philippine language, you can search for them in the search engines like Google. You may also get in touch with some companies and ask them for sample resumes in Philippines.Aside from that, if you need to take a refresher course or check out the current trends, you can also use the online materials as a reference when it comes to reviewing and applying the job resumes examples and samples in Philippines. They will help you a lot since you will not only be able to determine if the resume is good enough but also guide you in determining what details can be included in the resume.The job res umes examples and samples in Philippines can also be categorized into the broad category of Writing Skills. The main categories are Business, Human Resources, Engineering, Information Technology, Finance, Sales, and Management. The skills include Writing, Proofreading, Grammar, Networking, Presentation, Presentation Skills, Research, Writing Style, etc. But the good thing about these is that you do not need to have any specific skills for these skills; it all depends on your ability to meet the standards of the employer.In this way, if you can write a good story, you can even improve your skills in the same way. And if you don't have any stories to tell, you can always select any topic that fits your interests. You just need to know how to write the resume correctly. Once you get this down, you can choose the job resumes examples and samples in Philippines to enhance your skills.Remember that any skills will only get you so far if you do not know how to apply them. For example, if y ou have perfect writing skills but cannot express your ideas well, you can hire a ghostwriter who can do that for you. Aside from that, if you are looking for writing samples, you can also turn to your school library.At times, you will be lucky enough to see the outlines of books that the public school librarians have in their reading room. This is a great place to look for work sample resumes in Philippines. All you need to do is ask for them, and you will be given the list of sample resumes.By the way, if you want to avoid using any English words in your resume, you can also search for sample resumes in English. It will be easy for you since most of the English samples are easy to read and comprehend. You can also try the free resume services that you can avail by simply filling up the online forms. All you need to do is complete the required fields to be able to view the sample resumes.

Imperialism as a Source of Many Conflicts throughout History

Imperialism a source of most if not all conflicts throughout history. No matter the century or time period, the start of all wars, battles, and conflicts all draw their roots back to imperialism. Imperialism have had great impacts on nations and brought many changes to societies and their cultures. It has impacted on people lives and how they live. It has affected our societies to this day and shaped or helped create foundations for our countries and cultures that we have today. Imperialism began probably since humans could think and reasons, as the population of certain tribe, village, country, or colony increased then the people would try to imperialize other region and territories for their benefits. The new territory could provide with more resources and materials such as food and animals meat, gold for money and wood and trees to provide lumber for housing and buildings. The new imperialism started during the medieval ages from around the 15th and 16th centuries. The new imperia lism was not used as source of survival but to gain domination and power over everything and everyone around the world. During the 19th, the new imperialism changed the world drastically. Europeans established empires across the world. European nations were motivated by political needs that were associated with building empire’s national greatness and cultural diffusion. There expansion policy was aggressive. They wanted to promote the superiority of western society over a slow progressiveShow MoreRelatedThe Great War Of Archduke Franz Ferdinand1558 Words   |  7 PagesThe Great War, commonly known as World War 1, was a conflict between the years of 1914 to 1918, subjecting war between the Allied Powers which involved France, Russia, United Kingdom, Italy, Belgium, Serbia and the United States and the Central Powers, which consisted of Germany, Austria Hungary, Bulgaria and the Ottoman Empire. The Great War, remarked and ranked as the most deadly conflicts that have ever to occur in human history. Military and civilian deaths and wounded individuals resulted inRead MoreThe Impact Of British Imperialism On Modern India1244 Words   |  5 Pagesdid British Imperialism have an effect on shaping modern India? The main source that will be discussed in this paper is the seventh book of the Spotlight on History Series called The British Raj, which explains the reasons behind the conflicts between the British Empire and Indian nationalism and assess the achievements of a memorable relationship. The whole notion behind imperialism, more often than not, is regarded as a negative chapter in history, due to theRead MoreAppendix a American Imperialism1314 Words   |  6 PagesAssociate Program Material Appendix A American Imperialism Part 1 Complete the chart by identifying the following: Identify the countries or areas where the United States engaged in imperialistic actions during the period from about 1870 to 1914. Discuss why each area was important to American empire building—political, economic, and social. Explain America’s expansionist ideals. What were some factors that justified American imperialist actions? Identify the currentRead MoreWorld War 1-Who Was to Blame1174 Words   |  5 Pages1919 and Germany was fully held responsible for causing the war. But it is not justified, that Germany, and Germany alone, should be held responsible for the outbreak of the war, even though her military expansion, nationalism and imperialism have built tension throughout Europe. It was not only Germany who wanted to expand their empire or wanted to be the world power and also, the entangled alliance system was another cause of the war, and additionally, made the war ineluctable. Germany’sRead MoreThe Struggle Of The Asian Powers1173 Words   |  5 PagesHistory Final Essay Sulphur High School Timothy Gothrup 4/27/2015 â€Æ' Timothy Gothrup McNeese State University / Professor Mai Song HIST 122-RC: World Civilization from 1500 April 29, 2015 The Struggle of the Asian Powers During the â€Å"Age of Imperialism†, which lasted through the entire nineteenth century, most countries/ areas outside of North America and Europe struggled to adjust industry based economies. Asian countries were left with a dilemma: industrialize quickly or become subjectsRead MoreCesaire: The Discourse on Colonialism Essay1111 Words   |  5 Pagesable to elaborate on how he believes that race and class unite to dominate inferior subjects in nations throughout the globe. Throughout the essay, Cesaire provides reasoning for the socially constructed experiences of those dictated by colonial imperialism, particularly Africans, and proletarian conflicts in Europe. In such a way, Cesaire suggests a critical course of colonialism, imperialism, capitalism and communism – fundamental themes in political and intellectual agenda, and in the declarationRead MoreImperialism During The Late 1800s1658 Words   |  7 Pages The effects of imperialism during the late 1800s had been either the means of ruins of a society or the very means of success for a country. Due to the advanced technology, countries manipulated their new found power to the greatest potential. If not all, many industrialized countries expanded their rule over to foreign lands. As imperialism occurs within a country, the natives have the option to follow the new rulers or get destroyed. With inferior technology only such choice will result forRead More Fall of Man Depicted in Atwoods Backdrop Addresses Cowboy Essay1086 Words   |  5 Pages The sexual politics of the man-woman relationship, or more specifically the sexual exploitation of women by men, is a clear concern in Margaret Atwoods Backdrop Addresses Cowboy. Although the oppressor-as-male theme is by no means an original source of poetic inspiration, Atwoods distinction is that she views the destructive man-woman relationship as a metaphor for, symptom and symbol of, bigger things. From the vantage-point of feminine consciousness, Margaret Atwood empahsizes the backdropRead MoreThe Destruction Of Human Life1525 Words   |  7 PagesThroughout the history of human life, humans have strived for advancing themselves and society as a whole. It has taken only a few hundred years for humans to evolve from simple farmers into the powerful and successful people that they are today. Many sacrifices had to be made to get to this point, however some of them are overlooked by the majority of people. One of the most overlooked sacrifices is that of our environment. To const ruct the vast infrastructure that we all take for granted todayRead MoreThe Imperialism Of India By George Marshall1739 Words   |  7 PagesKai Middlebrook Mrs.McKnight World Literature: Per. 5 11 October 2015 British Imperialism in India â€Å"After three shots, the elephant still does not die. Orwell fires his two remaining shots into the elephant’s heart. He sends someone to get his small rifle, then pours ‘shot after shot into his heart and down his throat.’ Still, the elephant does not die. Orwell, unable to stand the elephant’s suffering and unable to watch and listen to it, goes away. The elephant, like the Burmese people, has become

What Makes an Epic Hero Essay - 1823 Words

What is an epic hero? Although we would like to believe that a hero would always be there to save us as needed, that is not always the case. All heroes are different, but what makes them epic? Many would answer that question by saying because they are in an epic poem or story; however, that answer isn’t true. An epic hero, of course is in an epic narrative, but it is what they accomplish in that specific text. First, an epic hero has to make a grand journey and be in the Gods favor or shall we say the chosen one. Also, he has to be seen as superior than any other man. For clarification, the epic hero needs to be bigger, faster, stronger, and wiser than anyone else in the story, except for the Gods of course. Also, and epic hero is also†¦show more content†¦However, his comrades are all relying on this great hero to win the war for them and he does eventually return to the battle field so he can be remembered and obtain glory as does any epic hero secretly wants. The a uthor John Keats agrees with me when he states in his article, â€Å"And he peoples it with towering heroes who thirst for honor and fight shattering wars.† (Keats, John). Another reason that qualifies Achilles as an epic hero is the fact that the Gods favor him throughout the poem because of his close relationship with them, but they usually do what is best for their hero; even if it means doing absolutely nothing, which they eventually do. Achilles is an epic hero that goes on a journey, and although he might not be the greatest hero, he is considered an epic hero. The next epic hero is Odysseus from the epic poem, The Odyssey which was also written by Homer. Odysseus isn’t your average guy, or even your average hero. His long journey begins ten years after the Trojan War starting off trapped on an island with a beautiful goddess. Andrea backs up my statement by asking the question, â€Å"He was sent off to in a land far away. Now the war is over, and he desperately wants to go home. Will the great warrior Odysseus find his way back?† (McAuliff, Coller, Andrea). He longs to journey home, but the goddess won’t let him, for she wants him as her husband;Show MoreRelatedWhat Makes A True Epic Hero?1313 Words   |  6 Pages What makes a true Epic hero? According to â€Å"From Legend to History† the main characteristics of an Epic hero would be that they would be fearless, larger than life, willing to defeat any evil creature and so much more. In the story of Beowulf all of these characteristics come to life in an epic battle with evil creatures in order to save a city and all the citizens inside of it. The story of Beowulf is set two places Geatland and Herot. The story begins long before Beowulf comes into theRead MoreAnalysis Of John Milton s Paradise Lost 1499 Words   |  6 Pageslight in Satan Epic heroism is a category that can pertain to many different subjects considering on what they have done or been through. No matter good, bad, or evil some of these subjects can find a way to make themselves that epic hero that best fits them. An epic hero goes by the definition of, a brave and noble character in an epic poem, admired for great achievements or affected by grand events. This being said can make any possible being, an epic hero if that being has done what is required.Read Morean analysis of the qualifications of an Epic Hero887 Words   |  4 PagesAn epic hero is someone who goes down in history as being brave, courageous, and obviously heroic. They don’t have to be asked to save the day, and often times, they aren’t even saving their own people. There are several epic poems that come to mind when thinking about European culture and where it came from. These stories are held as sacred to many different people, and will always be studied whenever the t opic of government or culture comes up. In these poems, or stories, there is always a heroRead MoreEpic Hero : William Shakespeare s Hamlet1606 Words   |  7 PagesEpic Hero. â€Å"A brave and noble character in an epic poem, admired for great achievements or affected by grand events† (Dictionary). The traditional characteristics of an epic hero includes being born into nobility, being decisive, being an excellent warrior who is respected, and ultimately winning in the end. In Shakespeare’s Hamlet, Hamlet fits the mold of an epic hero as he is born into nobility, but he breaks the mold of an epic hero as he never takes action against Claudius while in Othello,Read MoreAnalysis Of Byrons Poem1306 Words   |  6 Pages One such work is Byron’s, Don Juan. The poem follows the adventures of the Spanish hero Don Juan. He travels around the world and gets involved in bizarre situations, usually due to a romantic interest. The poem seems to share more similarities with the epic tradition than Wordsworth’s. Don Juan’s titular character, like many heroes in other epics, pre-existed the Byron poems. The story is vast in scale; it follows Don Juan’s adventures in love and travel. Juan’s adventures pull him from situationRead MoreThe Dark is Rising Series1373 Words   |  6 PagesIn every epic journey there are a series of events which the hero must go through if he is to become the ultimate hero. One of the most important parts in the epic journey is the descent into darkness or hell. Hell is a place where the hero travels to a dark place to learn about himself or to find very key information about his quest. However there is one common theme. The theme is the hero learns some valuable lessons. He or she learns how the underworld helps him to achieve the symbolic death andRead MoreThe Similarities Of An Epic Hero In Beowulf And Iron Man1183 Words   |  5 PagesPeople believe being a hero means saving the world, but that is not always true. In Beowulf and Iron Man, the use of theme and qualities of an epic hero are used to portray that they are similar and different. Three qualities that an epic hero must have are to be courageous, to have superhuman strength, and to be selfless. The theme in Beowulf and Iron Man is triumph because they both overcome challenging obstacles. The theme helps the reader in real life because that person will have to overcomeRead MoreChris Kyle : The Characteristics Of An Epic Hero871 Words   |  4 Pagespeople in the world, but they are never truly recognized as epic heroes. When people hear the word epic hero they think of the ancient warriors of Greece, Rome, and Troy or a story about other fictional persons/ animal s doing amazing things to help people. The veterans around us are the real epic heroes though. They often slip underneath the radar though because it can be hard to recognize someone who is truly humbled by their service. These epic heroes are the ones that willingly leave their familiesRead MoreSundiata Essay971 Words   |  4 Pages Epics Sundiata was written in Africa. Gilgamesh was the oldest one written. Other works include the Iliad and the Odyssey, which were written by the blind prophet Homer. What do all these works of literature have in common with each other? The similarity that all these works of literature have is that they are all epics. What does this mean? What is an epic? The definition of an epic is that an epic is a long, narrative poem with a hero that goes on a quest. What is the hero? Well, the characteristicsRead MoreHeroes and How to Represent Them929 Words   |  4 Pages The word hero is Greek, from a root that means â€Å"to protect and serve†. A hero is someone who is willing to sacrifice his own needs on behalf of others, like a Sheppard who will sacrifice to protect and serve his flock. At the root, the idea of hero is connected with self sacrifice† (Vogler, pg. 29). A hero represents an ego which distinct them from the rest of the human race. The journey of many heroes separates them from a family or tribe. They begin a long journey of only wisdom and power which

Essay about The Deeper Meaning of Christopher Marlowes...

The Deeper Meaning of Christopher Marlowes Doctor Faustus I do not agree with the frequently repeated comment that Doctor Faustus is an anti-intellectualist play that preaches that curiosity is dangerous. It is all too easy to see Faustus as the scholar, seeking knowledge, and his desire for knowledge that leads to his downfall. To confine the play to something so narrow is to ignore the deeper meaning behind the play. I believe that this deeper meaning is more important than the superficial idea that curiosity is wrong. I believe that the deeper meaning behind the play is the idea that in loosing sight of the spiritual level of existence, we loos sight of God. In doing so, we can no longer see Gods mercy and love, and so ignore†¦show more content†¦It has been said that power corrupts, and absolute power corrupts absolutely - this is what has happened to Faustus. He ceases to be the seeker of knowledge, but becomes a seeker of pleasure. One of the first things he wants is a wife: ... but leaving off this, let me have a wife, the fairest maid in Germany, for I am wanton and lascivious, and cannot live without a wife. Scene 5, lines 139-141 This marks the descent of Faustus from the intellectual seeking pleasures of the mind, to the hedonist seeking more sensual pleasures. It is not for being intellectual that we start to dislike Faustus, but for the numerous foolish and irrelevant displays of his power that he undertakes, and eventually his pride. This is exemplified by the sharp contrast between Faustus intentions at the beginning of the play, and the deeds he performs during the play: Ill have them read me strange philosophy, And tell the secrets of all foreign kings. Scene 1, lines 86-8 Instead of doing things like this, he squanders his power. It is his decision to indulge in, as Bentham was later to call them, lower pleasures that illustrates the falsity of the claim that Doctor Faustus is anti-intellectual. If this play were meant to be anti-intellectualist, then the scenes toward the middle of the play that involve Faustus attacking the pope, or summoning AlexanderShow MoreRelatedChristopher Marlowe Protests: The Moral of Doctor Faustus Essay883 Words   |  4 PagesWhen Doctor Faustus was written, there was turmoil in Elizabethan society. The old medieval view made God the most important aspect of the world, while mankind and the natural world were ignored. This was giving way to the idea that mankind and the natural world were supreme. At first glance, it seems that Doctor Faustus was written with the medieval ideal in mind, however, I believe this is not so. I believe that Marlowe subscribed to the renaissance view of the world, and Doctor Faustus was inten dedRead MoreWilliam Shakespeare s The Play Everyman 1275 Words   |  6 Pagesinvolved every person who’s ever thought about the meaning of life. Similarly, we can also see the shadow of a philosophical trace in Christopher Marlowe’s play, Doctor Faustus. The play personifies good and evil. The devil will fight for the soul of human beings, while our soul remain indecisive. Soon after, high lights appeared in the instance when the soul makes a decision. Even though both works are excellent, but I fell that Dr. Faustus made a bigger impact on me. Although both work revolvedRead MoreThe Tragical History Of Doctor Faustus1991 Words   |  8 Pagesâ€Å"The Tragical History of Doctor Faustus,† or â€Å"Doctor Faustus,† is a tragedy, written by Christopher Marlowe, which conveys the heavy use of symbolism and allusions to illustrate the conflict between good and evil during a time of rebirth in England. This play was first performed sometime between 158 8 and 1593, and published sometime in the early 1600s and is based on the protagonist, Faustus, who originates from a classic oral German legend. â€Å"Doctor Faustus† is one of the first of many publications

Fluorescent and Nonfluorescent Cytosolic †Free Samples to Students

Question: Discuss about the Fluorescent and Nonfluorescent Cytosolic. Answer: Introduction Wounds are common symptoms in skin caused by surgery or traumas. The coordinated process of wound healing restores functional barrier and epithelium integrity through blood coagulation, inflammation, in re-epithelialization caused by migration of keratinocytes, tissue formation by granulation and finally remodeling of the tissue. Integrins receptors play an essential role in all events of wound and scar healing by the forming and regulation cell adhesion (Weber et al., 2012). In this following report, we will design an experimental model in order to discuss the role of specific function-blocking integrin antibodies in healing and reducing the scars formed by burns through scarless invivo mechanism. During the process of wound healing the, cell interaction occurs with ECM molecules in the wound comprising the integrin receptors. Evidence has showed that integrins bind with various ECM molecules and alternately the various integrin heterodimers recognizes the ECM molecules management. Therefore, based on their specific overlapping and compensating functions, many integrin based knockout animals have displayed phenotypes of wound healing. Many extensive interactions are found in the different cells of wound healing. The major components that play a key role during the interactions are ECM proteins and integrins (Olczyk, Mencner Komosinska-Vassev, 2014). The functions of some of the specific integrin antibodies that play a vital role in wound healing are provided below: Integrin Expressed wound cells Ligands present in Wounds Cellular functions during wound healing Wound phenotypes present in animal models M2 Macrophages, NK cells, macrophages, neutrophils and T-cells Fibrinogen, plasminogen ICAMs, heparin, FN, LMs, COL I, uPAR, CCN1/Cyr6, CCN2/CTGF This integrin in mediates in leukocyte extravasations throughout the endothelium that promotes fibrinolysis and clears the fibrin clots aided by monocytes and neutrophils complied with uPar along with its ligand uPA Mediates the attenuation of deposited granulated tissue and re-epithelialization of wound found in Mintegrin knockout mice X2 Monocytes, dentritic cells macrophages and NK Fibrinogen, various ICAMs, COL I, OPN and heparin Involved in the mechanism of leukocyte extravasations No data available related to wound healing L2 Expressed in all leukocytes ICAMs, JAM-1 and lumican Causes the leukocyte extravasations throughout the endothelium No data available related to wound healing E7 T-lymphocytes and dentritic cells E-cadherin Also involved in leukocyte extravasations Involved in causing inflammation in skin lesions found in Eintegrin knockout model of mice but no data are available with respect to wound healing Source: (Koivisto et al., 2014) Thus, in order to understand the mechanism of the specific integrins tabulated above in the process of wound healing, an experimental model based on scarless in vivo is designed involved in healing the wounds due to burn. To experimental model was conducted through evaluation of three processes for proposing the animal model successfully in vivo such as indirect Immunofluorescence microscopy, Light microscopy and Western blot analysis management. In indirect immunofluorescence microscopy, light microscope combined with fluorescence microscope is used to identify the target molecules by visualizing the specificity of antibodies towards their antigen by marking with fluorescent dye such as fluorescein isothiocyanate (FITC) (Atale et al., 2014). Another procedure that will be implemented in this experiment will be through light microscopy which is used to magnify small samples with the use of visible light and lenses (Tomer et al., 2014). And finally, western blot analysis will also be done to successfully evaluate the experimental study. In western blot proteins are detected and analyzed. The proteins are separated from the gel into a membrane through the process of electrophoresis and are specifically visualized (Eaton et al., 2013). 2-month-old male and female C57BL/6 mice will be taken. The body weight should be around 2540 grams. The male and female mice should be housed individually. The animals should have access to free drinking water and standard chow. They should be maintained on a cycle of 12 hours light and darkness. The study will be carried out in strict accordance with the recommendations by the Australian Code of Practice for the Housing and Care of Laboratory Mice, Rats, Guinea Pigs and Rabbits. The protocol will be approved by Australianand New Zealand Society forLaboratory AnimalScience (Whittaker, 2014). A single surgery will be performed under Xylasine or Ketamine anesthesia, and all efforts will be maintained to reduce the animal suffering. In vivo scar healing experiment Even burn wounds will be ensured by shaving off the hair on the dorsum. The dorsum is considered as an ideal choice because it becomes difficult for the mice to reach the wound area and create further injuries in that region. The mouse will be placed on its back in a plastic frame template, and a window will expose a predetermined skin surface area. The exposed area from the template will be immersed in a water bath at 100C for 8 seconds. This will inflict a thickness burn (Domergue, Jorgensen Nol, 2015). The mice will be observed for any pain or discomfort signs and will be treated with buprenorphine if required. The temperature of water bath and the exposure time can vary. The healing process will be monitored and evaluated for many days during the first week under a slit lamp and then once every week. Unwounded skin from identical locations of WT and FMOD/animals were collected as controls. Tissue samples for histology will be bisected between two 40 nylon sutures and they will be fixed in 10% formalin (Wosgrau et al., 2015). After fixation, the samples will be dehydrated. They will be embedded in paraffin followed by and 5-m section cuts for HE staining. Indirect Immunofluorescence Microscopy For frozen sections, the tissues will be frozen in OCT, 5m sections will be cut.Indirect immunofluorescence (IF) will be performed. The primary antibodies will be used on the mounts incubated at 4C overnight: M2, L2 and E7. Secondary that is conjugated to either rhodamine or fluorescein will be used with blocking conditions. The incubation period of the sections will be 1 hour in room temperature. Donkey antihuman IgG or donkey antirat IgG will be used (Stelzer, 2015). Sections will be cover slipped DAPI mounting media followed by examination under a confocal microscope or fluorescence microscope with a digital camera. We will run negative controls where there was omission of primary antibodies. We will observe at least 3 tissues per antibody. The captured images on the confocal microscope will be evaluated with software for image analysis like Media Cybernetics, Bethesda, MD or Image Pro Plus v.7. The tissues will be fixed either in 4% paraformaldehyde for HE (hematoxylin-eosin) or Karnovsky's with strength for TEM (transmission electron microscopy). For TEM,the scarred tissues, 6090 ? thick will be cut on ultramicrotome and examined under an electron microscope. For HE, we will cut and stain 6-m sections. The sections will be examined under a light microscope that has a digital camera fitted with it. Western Blot Analysis Epithelial tissue will be scraped from the mice after scarring it in by using a water bath following euthanization management. The tissues will be subjected to flash freezing in liquid nitrogen. Furthermore, they will be processed for Western blot analysis. Eight sections of tissues will be used for each experiment. Tissues will be homogenized and lysed. Protein will be subjected to purification and assay using Biorad; Hercules, CA. Total proteins will be loaded in equal amounts on a nonreducing Tris-glycine gel (4%20%). The proteins will be transferred to an Immobilon-P or Millipore membrane which will be stained with Sigma or Ponceau S. This will check transfer efficiency (Taylor Posch, 2014). Membranes will be blocked with TBS containing 5% milk and probed with the primary antibodies M2, L2 and E7and will be incubated at 4C overnight. These membranes will be incubated with a secondary antibody that is conjugated with HRP at room temperature for 1 hour: donkey antihuman IgG (M2) o r donkey antirabbit IgG (E7). Chemiluminiscence with Millipore will be used to visualize immunoreactive bands. The same procedure will be repeated thrice. ImageJ v.1.5 software will be used to measure the intensity of the bands. Prism 5.0 will be used to plot the fold enhancement values. Conclusion Thus from the following discussion, an experimental design can thus be constructed in order to study the underlying mechanism of some specific integrins involved in improving the healing and thereby reduce the scar formed in burns. From this experiment it can be detected whether the specific integrins have been upregulated inside the epithelium during the healing of burn scars in mice. This will provide evidence for understanding the mechanisms of excessive scarring and wounds. It will help to design novel therapeutic approaches and interventions that will utilize integrins as targets for scar reduction. References Atale, N., Gupta, S., Yadav, U. C. S., Rani, V. (2014). Cell?death assessment by fluorescent and nonfluorescent cytosolic and nuclear staining techniques.Journal of microscopy,255(1), 7-19. Domergue, S., Jorgensen, C., Nol, D. (2015). Advances in research in animal models of burn-related hypertrophic scarring.Journal of Burn Care Research,36(5), e259-e266. 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